rat trpv4  (Addgene inc)

Journal: Cells

Article Title: Generators of Pressure-Evoked Currents in Vertebrate Outer Retinal Neurons

doi: 10.3390/cells10061288

Figure Lengend Snippet: The expression of several MSCs in the salamander retina. Confocal images show the retina slice triple-labeled for calbindin D-28k (Calb), calretinin (Calr), and TRPV2 ( A , E , F ) or TRPV4 ( B ) or probed for TRAAK ( C ). ( A ) TRPV2 antibody brightly labels the outer plexiform layer (OPL), half disks in the rod outer segment (OS) (white arrow), and cone OS (black arrow) and weakly reveals some processes in the inner plexiform layer (IPL) and somas in the ganglion cell layer (GCL). ( B ) Bright TRPV4-immunoreactive puncta are mostly present in the OPL and terminals of cones (black arrow, ( b5 )), while weaker TRPV4 signals are visible in somas and dendrites of horizontal cells (HCs, ( b1 )), bipolar cells (BCs) ( b2 ), the basal membrane of rods and putative rod axon terminals (white arrow, ( b3 , b4 )), the inner plexiform layer (IPL), and somas in the GCL. ( C ) TRAAK antibody labeled the OS of single cones (black arrow) the most brightly, and it clearly revealed the OS of rods (white arrow). Weaker TRAAK signals are sparsely present in the OPL and IPL. ( D ) Calretinin antibody heavily labeled HCs and clearly labeled some BCs. ( E ) Calretinin-labeled soma of HCs positive for TRPV2. ( F ) At the OPL focal plane, nearly half dendrites of HCs that are identified by calretinin ( F2 ) and GABA (( F3 ), red) are labeled for TRPV2 ( F1 ) (white double-arrow). ( F1 , F2 ) display the blue and green channels of ( F3 ), respectively. OSL: outer segment layer; ISL: inner segment layer; RGC: retinal ganglion cell. Scale bars: 20 µm.

Article Snippet: It was raised against rat TRPV4 (Q9ERZ8, aa853-871, peptide immunogen sequence: CDGHQQGYAPKWRAEDAPL).

Techniques: Expressing, Labeling, Membrane

Journal: Cells

Article Title: Generators of Pressure-Evoked Currents in Vertebrate Outer Retinal Neurons

doi: 10.3390/cells10061288

Figure Lengend Snippet: The effect of modulators of the mechanical sensitive ion channels on the light response of rods. Rods were recorded for the light response in current-clamp mode. ( A , B ) Three to four rods were simultaneously recorded with the multi-cell patch-clamp technique. TRPV2 agonist 2APB weakly depolarizes rods and slightly reduces the light response ( A ). The K + channel blocker TEA largely depolarizes rods and reduces the light response. ( B ) TRPV4 channel agonists GSK and 4aPDD slightly hyperpolarize rods (the highlighted trace). ( C ) The light response in a rod was largely and reversibly reduced by ruthenium red (RR). ( D ) A blocker for BK channels, IBTX, enhances the light response of a rod. The two traces in each panel in C and D were recorded 2–3 min apart. ( E ) The light-evoked potentials recorded at eight light intensities are larger at 23 °C than at 31 °C and severely disrupted at 43 °C. ( B – E ) Membrane potentials were maintained at ~−40 mV.

Article Snippet: It was raised against rat TRPV4 (Q9ERZ8, aa853-871, peptide immunogen sequence: CDGHQQGYAPKWRAEDAPL).

Techniques: Patch Clamp, Membrane

Journal: Cells

Article Title: Generators of Pressure-Evoked Currents in Vertebrate Outer Retinal Neurons

doi: 10.3390/cells10061288

Figure Lengend Snippet: Mechanosensitive channels in the outer retina.

Article Snippet: It was raised against rat TRPV4 (Q9ERZ8, aa853-871, peptide immunogen sequence: CDGHQQGYAPKWRAEDAPL).

Techniques: